The maize stalk of the third internode in the maturity stage is used as the research material.The research uses four ways,such as free-hand sectioning,frozen sectioning,paraffin sectioning,thin sectioning and compares the quality of the slice of different methods.This research lays the technical foundation for studying the relationship between the structure of maize stalks and lodging.The results show that free-hand sectioning method for getting the structure slice of mature maize stalks is a relatively convenient and rapid method,and the area of slice is relatively big.The slice is suitable for a wide range of observation and statistics.Frozen sectioning is a fast way to get the slice of mature maize stalks,and the area of slice is relatively small and the slice is suitable for a small range of observation.Thin sectioning is a good way to get perfect slice.It fits for high multiple microscope observation and small range of SEM observation to observe tissue structures,but the slice area is too small.Paraffin sectioning is not suitable for getting the structure slice of mature maize stalks.The research suggests that free-hand sectioning method is the most suitable way for observation and study of the structure of mature maize stalks.