Cloning and Expression Analysis of MSOC1 Gene in Mango (Mangifera indica L.)
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    Abstract:

    MADS-box transcription factor plays a crucial role in plant development,especially controlling the formation and development of floral organs.In order to investigate the role of MADS-box transcription factor in mango flower development,we cloned the flower-specific gene from mango inflorescence,named MSOC1 (GeneBank accession no.KP404094) with homology-based cloning and RACE method.The open reading frame of MSOC1 was 733 bps,encoding 223 amino acids with molecular weight 25.6 kD and isoelectric point 8.96.Sequence alignment and phylogenetic tree analysis indicated that the MSOC1 deduced protein contained a conservative MADS-box and semi-conservative K domain and belonged to the SOC1/TM3 subfamily of the MADS-box family.Tissue-specific analysis showed that MSOC1 was expressed in various tissues of mango,high expression in stems,leaves and inflorescences,low expression in roots and flowers.

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WEI Junya, TANG Jie, LIU Guoyin, LIU Debing, CHEN Yeyuan. Cloning and Expression Analysis of MSOC1 Gene in Mango (Mangifera indica L.)[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(6):1092-1097

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  • Online: August 09,2015
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