‘Wink’ (fertile),‘Zhongshanhong’ (male sterile),‘Wink seedling-3’,‘Wink seedling-12’ were examined.The flower characteristics,pollen viability,pollen shape and the cytological features of microspores were observed,DYT1,TDF1 and MYB4 genes that was related to the tapetum development were analyzed.The semi-sterile characteristics of the ‘Wink seedling-3’ and ‘Wink seedling-12’ were identified preliminarily,and the pattern of the gene regulation of pollen abortion was studied.The results indicated that:(1)the main features of two ‘Wink seedling’ grapes:filaments curl and shorter than the style,few pollen in the anther,the pollen viability was only about 10%.There were two kinds of pollen grains in anthers:normal prolate spheroidal pollen grains and approximately spheroidal pollen grains.(2)The anther wall structure was not obvious,and the microspore mother cells were few and loose in the ‘Wink seedling’ grapes.The tapetum of ‘Wink seedling-3’ did not degrade completely in the binuclear stage,the nutrients of tapetum could not supply for the development of spores timely,which caused the abortion of microspores in the late developmental stage.The tapetum of ‘Wink seedling-12’mostly disintegrated at the early mononuclear microspore,which caused the abnormal supply of nutrient and the abortion of microspores.(3)In the meiosis stage,DYT1 and TDF1 expression in ‘Wink’ were the highest,MYB4 was the lowest;These three gene expression in ‘Zhongshanhong’ were contrary to ‘Wink’,and the levels of two ‘Wink seedling’ grapes laid between ‘Wink’ and ‘Zhongshanhong’.After the mononuclear microspore stage,three gene expression in ‘Wink’ were always low,DYT1 expression levels were the highest,TDF1 and MYB4 were the lowest;TDF1 and MYB4 expression levels in ‘Wink seedling-3’ were highest in the mononuclear microspore stage,and TDF1 expression in ‘Wink seedling-12’ was highest in the binuclear stage.The study showed that ‘Wink seedling-3’abnormal tapetum degradation was probably related to the DYT1 insufficient expression in the meiosis stage,the TDF1 overexpression in the mononuclear microspore stage,and MYB4 unusual expression in the mononuclear microspore stage.‘Wink seedling-12’abnormal tapetum degradationwas probably related to the DYT1 insufficient expression and MYB4 overexpression in the meiosis stage,and the TDF1 overexpression in the binuclear stage.