In this study,we integrated the mapping information based on collecting of positioning information about QTLs related to cadmium accumulation in soybean grains.Then the recombinant inbred line(RIL) population(F_6:7) derived from ‘Zhonghuang24（ZH24）’ and ‘Huaxia3(HX3)’ were used to validate one major QTL Cda1/Cd1 which controlled the cadmium accumulation in soybean grain.The results showed that the major QTL Cda1/Cd1,which were mapped by two groups,actually located in the same fragment on chromosome 9.The point mutation in GmHMA1,that one of the candidate gene for Cda1,was similar in ZH24 and HX3.The Cda1 was not linked with the differences of cadmium concentration in various organs between ZH24 and HX3.The findings indicated that there were other new QTLs controlling the difference of cadmium accumulation in grain between ZH24 and HX3.It is necessary to make genome-wide scanning and identification in future.