Full cDNA of one MADS-box gene,PrseSHP with GenBank accession No.GU362645,was cloned from Prunus lannesiana using homologous cloning and RACE method.The full length of PrseSHP cDNA is 1 223 bp，containing an open reading frame（ORF）of 741 bp and coding for a polypeptide of 246 amino acid residues.Sequence and phylogenetic analyses grouped PrseSHP into PLE/SHP lineages of the MADS-box family.Conceptual translation revealed that PrseSHP contain MADS,I,K and C domains.Expression analysis suggested that PrseSHP expressed mainly in petal,stamen,gynoecium and young fruit of P.lannesiana ‘Makino’.Moreover,functional analysis suggested that transgenic Arabidopsis was obviously dwarf,and flowering during 6-8 rosette leaves,which was early than that of wild-type Arabidopsis who was flowering during 14-17 rosette leaves.Ectopic expression of PrseSHP could obviously promote flowering of transgenic Arabidopsis.Our results suggest that PrseSHP are involved in flowering in Cherry Blossom.