The gene FtAH4L,which encoding the autoinhibited H⁺-ATPase isoform 4 like(AH4L) involved into flavonoids transportation,was cloned from tartary buckwheat cultivar Xiqiao No.2(Fagopyrum tatarium).The length of FtAH4L gene was 3 398 bp,and the ORF(Open Reading Frame) was 2 898 bp encoding 966 amino acids.The molecular weight of FtAH4L was 109 kD,and its pI was 6.48.Conserved motifs and phylogenetic analysis demonstrated that AH4Ls are relatively conservative in different plant species.At the seedling stage of tartary buckwheat,semi-quantitative RT-PCR analyzed the expression profiles of FtAH4L gene,and AlCl₃ colorimetric method measured the total flavonoids content in cotyledons and hypocotyls,after jasmonates treatment and different lighting(White fluorescent,LED White,LED Blue,LED Red and UV-B).The results showed that FtAH4L expression and flavonoids content not only had a significant increase(P<0.05),but also showed a positive correlation in cotyledons and hypocotyls after jasmonates treatment.Under all the lightings,FtAH4L expression was unremarkable change(P>0.05) while flavonoids content was raised obviously in cotyledons.However,in hypocotyls,FtAH4L expression and flavonoids content were promoted notably(P<0.05) except LED Red.Particularly,LED blue and UV-B presented the extremely significant affect(P<0.01).This work could provide an important reference to understand the mechanism of FtAH4L gene involved in flavonoids transportation in tartary buckwheat.