The F₂ population was derived by crossing two diverse Cucurbita maxima lines ‘98-2-351’ and ‘06820-1’.Investigation and statistical analyses were conducted on rind and flesh color of mature fruits for parental plants and each generation.The results demonstrated that the orange and grey rind plants in F₂ population were fitted for the ratio of 3∶1,indicating that the grey rind was controlled by a single recessive gene;similarly,the yellow and white flesh plants in F₂ population were also fitted for the ratio of 3∶1,showing that the white flesh was controlled by a single recessive gene.With bulked segregation analysis and recessive-class approaches,two SSR markers PU078072 and PU013839 were detected to be tightly linked to the locus CmRc at a distance 5.9 and 14.5 cM,respectively.Meanwhile,one SSR marker PU132712 was founded to be closely linked to the locus CmFc at a distance 6.7 cM.This study laid the foundation for further screening the molecular markers closer linked to rind and flesh color genes of C.maxima and locating them accurately.