With Lycium barbarum L.cv.Ningqi No.1 as material,using high performance liquid chromatography (HPLC) technology,we determined different development period of L. barbarum L.fruit sugar (Fructose,Glucose,Sucrose and Starch) content in different regions (Yinchuan in Ningxia,Baiyin in Gansu and Delingha in Qinghai).At the same time,we monitored the meteorological factors during development of fruit in different regions to discuss the effect of main meteorological factors on sugar accumulation in L. barbarum L.fruit.The results showed that:(1) During the fruit development period of L. barbarum L.,the mean temperature and the temperature difference between day and night of different regions presented the change trend of Yinchuan>Baiyin>Delingha,while the mean light intensity presented YinchuanL. barbarum L.fruit was given priority to with accumulation of hexose (glucose and fructose),sucrose and starch content rarely.The content of hexose in the ripe fruit was in the order of Delingha>Yinchuan>Baiyin.(3) The meteorological factors that affected sugar content of L. barbarum L.fruit in Yinchuan region mainly were mean temperature,daytime mean temperature and mean temperature difference,and in Baiyin region mainly were mean temperature difference,temperature during the day and mean light intensity also had a certain influence,while in Delingha region mainly were mean temperature difference,mean light intensity and daytime mean temperature.This research suggested that higher mean temperature and mean temperature difference in Yinchuan and stronger mean light intensity and longer light hours in Delingha were conducive to L. barbarum L.fruit hexose accumulation.Meteorological factors limited the sugar accumulation of L. barbarum L.fruit mainly were bigger relative humidity and weaker light intensity in Baigin region.