Using RT-PCR technology,we cloned the R2R3 MYB gene LbMYB103 cDNA fragment,which contains a complete open reading frame(ORF),from wolfberry variety ‘Ningqi No.1’.The base sequence was entirely consistent with that of the known gene HQ415755.The plant over expression vector pMDC83-LbMYB103-GFP was constructed with Gateway Technology and confirmed by enzyme digestion and sequencing.We transformed pMDC83-LbMYB103 into onion epidermal cells using particle bombardment and found that LbMYB103 was located in the nucleus.We used the real-time quantitative PCR approach to analyze the expression pattern of LbMYB103 in various organs,and the result showed that expression of LbMYB103 was the highest in flower and anther,relatively lower in fruits,but undetectable in leaf,stem and root,indicating that LbMYB103 may play some roles in wolfberry reproductive organ process,such as anther development.We transformed pMDC83-LbMYB103 into Arabidopsis thaliana（Col-0）using Agrobacterium-mediated method.After plant selection and regeneration,a total of 52 and 29 regenerated T₁ and T₂ plants with resistance were obtained respectively.The PCR detection showed that a total of 41 T₁ plants and 23 T₂ plants containing LbMYB103 gene,and the real-time PCR analysis proved that LbMYB103 gene in transgenic A.thaliana could conduct normal expression.The phenotypic observation showed abnormal anther and flower development,shorten pods without seed both in T₁ and T₂ transgenic A.thaliana,further indicating LbMYB103 gene might be related to the plant fertility.The results lay a foundation for further study on the LbMYB103 gene,which may play a regulating role in the process of wolfberry anther development.