In this study,we cloned cDNA and DNA sequences of anthocyanin synthase (ANS) gene,named MaANS,based on transcriptome sequencing in Muscari armeniacum.We did real-time fluorescence quantitative analysis about MaANS.While taking advantage of chromosome walking.We cloned a 1 044 bp upstream sequence of MaANS.Bioinformatic analysis showed that MaANS contains a 1 065 bp open reading frame,encoding 355 amino acids.The consistency of cloned DNA and cDNA is 89.62%,DNA sequence inserts a 79 bp intron from 515 bp to 594 bp on downstream of ATG.TATA-box is at -70 bp of the promoter which has a plurality of light-responsive elements and MYB binding sites.Real-time fluorescence quantitative analysis showed MaANS was predominant expression in flower and the expression level at fully colored stage was the highest.In this study,gene cloning and expression analysis on MaANS are important to in-depth study the function of MaANS gene and analyze the colored mechanism of Muscari armeniacum.