The full length cDNA of a sHSP17.3 was identified by analysis of the transcriptome of Juglans regia,which was named as JrsHSP17.3.The ORF of JrsHSP17.3 is 474 bp in length and encodes a 17.64 kDa protein with 157 amino acid residues and the theoretical pI of 5.35.To understand the tissue specific and temperature responsive expression profiles,we analyzed the transcription levels of JrsHSP17.3 in roots,stems and leaves exposed to heat and cold stresses at different time points by quantitative real time PCR (qRT-PCR).The results showed that JrsHSP17.3 was expressed more or less in stems,roots and leaves,and was obviously induced after high (36 ℃-52 ℃) and low (16 ℃-6 ℃) temperature treatments,among which the highest expression level was 142.02-fold of control under 12℃ for 1 h,suggesting JrsHSP17.3 could response to heat and cold temperature stresses.These results will provide a basis for further understanding the function of JrsHSP17.3 in cold and heat tolerance.