The object of this study was to identify and characterize the haplotypes of Vp-1A in Tritivum urartu by PCR and semi-quantitative RT-PCR and their phylogenetic relationship with other wheat related species.In total,4 novel Vp-1A haplotypes were explored among a collection of 20 T.urartu genotypes and designated as TuVp-1Abgi,TuVp-1Acgh,TuVp-1Adfi and TuVp-1Aefi,respectively.The main differences between TaVp-1Aa (AJ400712 ) in common wheat and the newly identified haplotypes were the numbers of TTC repeat,the deletions and SNPs in second and fifth introns.In addition,the expression levels of Vp-1A homologues were different in genotypes harboring these 4 haplotypes.Furthermore,different mRNA levels among the four genotypes were observed after ABA treatment,indicating their different responsiveness to ABA exposure.These results suggested that the indels in the second,third and fifth intron regions of the Vp-1A gene affected its expression and its sensitivity to ABA,and thus seed dormancy.Therefore,our study characterized the Vp-1A haplotypes in a set of T.urartu germplasm and identified a novel haplotype TuVp-1Adfi which might be useful in wheat improvement for PHS resistance.