This experiment was conducted with Clerodendrum trichotomum Thumb pollen.The effects of the storage mode,storage time and storage temperature,thawing way and precool way on C.trichotomum Thumb pollen germination were explored.In addition,the changes of the protective enzyme activities and osmoregulation substances content were estimated under -80 ℃ and 4 ℃low temperature storage process.The results indicated that:(1)the effect of two pollen storage ways with anther and powered at low temperature condition was not significant.The different precooling ways have no significant differences on the effect of low temperature storage.37 ℃ bath thawing is significant better than room temperature thawing for pollen germination under -80 ℃.(2) Germination rate of pollens still maintained at 7.99% after three months under -80 ℃,having improved the pollen germination more effectively.(3) With the low temperature stress counting,the activities of SOD and POD increased at the beginning then decreased under -80 ℃ and 4 ℃.(4)The content of soluble sugar under -80 ℃ and 4 ℃ and soluble protein under -80℃ increased at the beginning and then decreased;The content of soluble protein under 4 ℃ decreased continually.The study found that C.trichotomum Thumb pollen was subjected to be storage at -80 ℃.The pollen improved the cold resistance and contain its activity by enhancing the SOD activity,POD activity and soluble sugar content,soluble protein content when it was subjected to low temperature stress.