Characterization and Expression Analysis of MaTPS1 from Banana
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    Abstract:

    A fulllength MaTPS1 of 3 946 bp from banana genome A was obtained.It contains a complete open reading frame of 2 562 bp length,encoding 853 amino acids.Biological information analysis showed that MaTPS1 protein belongs to the unstable protein,with an isoelectric point of 4.72 and two domains TPS and TPP.Sequence analysis prediction indicated MaTPS1 is a hydrophobic transmembrane protein without signal peptide and localized in cytoplasm.The amino acid sequence of MaTPS1 protein has 74.81% homology to the amino acid sequence of other known plant TPS ,with 100%,79.91%,71.33%,63.93% and 65.13% to two varieties of Musa acuminata subsp.malaccensis,Zea mays,Camellia sinensis,Coffea canephora.Organspecific analysis suggested that MaTPS1 is expressed in banana roots,corms,false stems,leaves,flowers and fruits,but with higher level in roots,corms and flowers.The banana plantlet was treated with ABA,ACC,drought,cold,salt and banana fusarium oxysporum f.sp stress,qRTPCR analysis showed that the expression of MaTPS1 increased under salt stress,and peaked at 24 h of treatment,while decreased under other biotic and nonbiotic stress,which suggested MaTPS1 may play a role in regulating salt resistance and enhance salt resistance of banana.

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XU Guiying, XU Biyu, XING Wenting, JIA Caihong, WANG Zhuo, CHANG Shenghe, WANG Anbang, SHU Haiyan, JIN Zhiqiang. Characterization and Expression Analysis of MaTPS1 from Banana[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(10):1934-1940

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  • Online: November 02,2015
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