In this study,a gene encoding the CBF/DREB transcription factor was isolated from cotton（Gossypium barbadense L.）with PCR based on bioinformation databases.Sequence analysis showed that the fulllength of GbCBF6 (GenBank accession No.KR233255)was a 753 bp open reading frame which encoded a protein of 251 amino acids with predicted molecular weight of 27.82 kD and a isoelectric point of 7.68.Multiple sequence analysis showed that GbCBF6 protein share high similarity with those plant cold stressrelated CBF proteins in other plants,which contain the classic AP2 domain and two CBF signature sequences.There are dramatic amino acid sequence differences between GbCBF6 and four reported cotton GhCBFs.Phylogenetic analysis revealed that the isolated cotton GbCBF6 gene can be classified into A1 subgroup of the DREB subfamily.RTPCR analysis indicated that the expression of GbCBF6 gene was downregulated by dehydration,but was upregulated by cold stress.Under high salinity,the expression level of GbCBF6 gene decreased firstly,and then increased at 6 h after treatment.In summary,these results indicated GbCBF6 may play important roles in response of cotton plant to abiotic stresses.