Abstract:Effect of exogenous spermidine(Spd)on growth and chlorophyll precursors content of spinach plants under NaCl stress was studied by hydroponics using ‘cv.Quanneng’ of spinach,a sensitive cultivar to salt.The results showed that:(1)growth of spinach plants was inhibited significantly by 250 mmol·L-1 NaCl,as a result of total fresh weight,total dry weigh,shoot fresh weight,shoot dry weigh,root fresh weight and root dry weigh of spinach plants were significantly decreased by 57.23%,53.08%,63.14%,55.05%,42.22% and 42.86%,respectively,as compared with control.However,plant growth was enhanced by 1.0 mmol·L-1 Spd,those indexes increased by 62.83%,71.19%,60.57%,71.74%,70.31% and 69.23%,respectively,as compared with salt stress treatment.(2)Chlorophyll a(Chl a),chlorophyll b(Chl b)and total chlorophyll[Chl(a+b)] contents in leaves of spinach plants were decreased by 250 mmol·L-1 salt stress to 42.31%,54.55% and 44.53%,respectively,but those were increased by 1.0 mmol·L-1 Spd to 46.24%,51.85% and 47.11%,as compared with salt stress treatment.(3)Chlorophyll precursors such as Chl a,Chl b,protochlorophyll(Pchl),Mgprotoporphyrin Ⅸ(Mgproto Ⅸ),protoporphyrin Ⅸ(Proto Ⅸ)and uroorphyrinogen Ⅲ(Uro Ⅲ)were decreased by salt stress,but were increased by Spd.However,others such as δaminolevulinic acid(ALA)and porphobilinogen(PBG)were enhanced by salt stress,but were suppressed by Spd.These results suggested that chlorophyll synthesis process from PBG to Uro Ⅲ was inhibited by salt stress,and this inhibition was alleviated by exogenous Spd,as a result of chlorophyll increasing.