In this study,the CsNAC1 and CsNAC2 genes,which encode the NAC transcription factors,were cloned from Camellia sinensis cultivar ‘Yingshuang’ by RT-PCR based on the tea transcriptome database.(1)Sequence analysis indicated that the open reading frame of CsNAC1 is 1 044 bp,which encodes 347 amino acids;and the CsNAC2 gene is 1 047 bp,which encodes 348 amino acids.Domain prediction and multiple alignments of CsNAC1 and CsNAC2 showed that N-terminals of the proteins contain typical NAM conservative structure which belongs to NAC family.(2)Phylogenetic analysis showed that the CsNAC1 and CsNAC2 belong to NAP and AtNAC3 subgroups of NAC family,respectively.(3)Molecular modeling showed that the CsNAC1 and CsNAC2 proteins contain 3 and 2 α-helix,6 and 7 β-strands,respectively.(4)Expression profiles of CsNAC1 and CsNAC2 genes in different tissues of two tea cultivars ‘Yingshuang’ and ‘Anjibaicha’ and under different temperature stresses were detected by quantitative real-time PCR.Results showed that the expression profiles of CsNAC1 gene between two tea cultivars exhibit similar tissue specificity and both the highest expression levels were found in mature leaves;while CsNAC2 gene showed the highest expression levels in the tender leaf of ‘Anjibaicha’ and root of ‘Yingshuang’,respectively.Under high (38 ℃) and low (4 ℃) temperature treatments,the expression profiles of CsNAC1 and CsNAC2 genes were different between the two tea cultivars and processing time,and were also influenced by high and low temperature stress treatments.