This research used strawberry ‘Benihoppe’(Fragaria ananassa ‘Benihoppe’) as test materials,an ABA receptor gene FaPYL9 was isolated from strawberry fruit,it contains an open reading frame of 555 bp nucleotides,encoding 184 amino acid sequence and contains an amino acid conserved region PYR_PYL_RCAR_like.FaPYL9 gene expression in seven periods of strawberry fruit development showed that with strawberry fruit development FaPYL9 gene expression rapidly increased,and had the highest expression in the fruit full red stage.FaPYL9 gene RNA interference in strawberry fruit can delay the strawberry fruit ripe for 3 to 5 days.Meanwhile FaPYL9 gene RNA interference to reduce expression of fruit color related genes FaCHS and FaUFGT,sucrose and anthocyanin contents in fruit decreased,ABA content and fruit hardness increased.Results showed that the FaPYL9 gene plays an important role in the strawberry fruit development,FaPYL9 gene can promote strawberry fruit ripening.