Abstract:According to transcriptome data of Fagopyrum tataricum at flowering stage,we cloned a bHLH transcription factor gene and designated as FtbHLH4.The expression patterns of FtbHLH4 under abiotic stresses were determined by qPCR.The results showed that the DNA sequence of FtbHLH4 was 1 852 bp in length and contained 7 exons and 6 introns,and in line with the principle of GT-AG splicing principle.The cDNA of FtbHLH4 contains a 1 062 bp ORF,which encoded 353 amino acids residues,and a conserved Helix-Loop-Helix domain.The expression of FtbHLH4 was up-regulated under ABA,NaCl and PEG stresses.After being treated with ABA,NaCl or PEG for 48 h,the expression level of FtbHLH4 increased to the maximum,which was 11.3,12.0 and 6.1 folds of the control,respectively.However,the expression of FtbHLH4 was down-regulated under cold and UV-B stresses.The expression level decreased to 24% and 23% of the control after 6 h and 12 h stress treatments,respectively.These results suggested that FtbHLH4 participate in the response process of F.tataricum under the abiotic stresses in different mechanisms.