At the present study,the binary insect-resistant BmkIT-Chitinase gene was successfully transformed into the Chinese cabbage inbred line 20-19-3 via pollen mediated transformation approach assisted by supersonic.Five transgenic Chinese cabbage inbred lines Z1-5,Z2-7,Z9-6,Z11-6 and Z20-13 were obtained.The genetic rules,transgenic expression and insect resistance of above transgenic materials were compared with untransformed control.The PCR amplification and Southern blot hybridization detection on transgenic plants and their progeny showed that:(1)BmkIT-Chitinase gene has been transformed into the genome of receptors and the transgenics can inherit stably.Most transgenics exist in multi-copy form and single copy transgenics appeared in few translines.(2)ELISA assay verified the expression of foreign gene and the expression product of BmkIT-Chitinase gene in T₄ transgenic plants were high to 0.069 μg·g^-1 fresh leaves.(3)Field insect-resistant bioassay showed that there are significant differences on insect resistance between transformed inbred lines and their control groups.The level of insect resistance activities of Pieris rapae Linnaenus and Plutella xylostella Linnaenus enhanced 2-3 degree.