Tropane alkaloids,including atropine,hyoscyamine,anisodamine and scopolamine are important anticholinergic drugs.Insight into molecular regulation mechanism of biosynthesis on tropane alkaloids in some Solanaceae herb and developing plant bioreactor to improve tropane alkaloids producing,especially scopolamine is becoming a hot pot in recent years.In this review,we summarized progress on tropane alkaloids biosynthesis tissues,molecular regulation and bioreactor technology.In addition,we discussed some problems in this field and looked forward its development in the future.