GGB is a negative regulator of drought resistance. In order to obtain the Arabidopsis ggb mutant, we used AtU6 promoter to drive the expression of AtGGB sgRNA and coresponding CRISPR/Cas9 genome edting vector was constructed and was transferred into Arabidopsis by Agrobacteriummediated floral dip. After sequencing of GGB in T₂ generation, two kinds of mutants with a deletion of 4 bases and an addition of 1 base (T) were found at the target site, respectively. Semiquantitative RTPCR analysis results showed that the expression of GGB gene was almost not detected in the ggb mutant, which indicated that the mutants were GGB knockout lines. Through measuring the water loss rate, drought resistant and seed yield per plant, ggb mutant exhibited decreased water loss rate, improved drought resistance, but unchanged seed yield per plant compared to wildtype. Taken together, the above results indicated that GGB is an ideal candidate target gene for crop molecular breeding and ggb mutant is useful for functional complemention of GGB homologous genes cloned from crops.