To obtain bluish Lilium spp. in direct breeding, we screened the suitable varietyOT lily Robina for genetic transformation. Here, both embryonic callus induced from filament and regenerated bulb scales of OT lily Robina were used as the transformation material. Agrobacteriummediated transformation of Phalaenopsis F3′5′H was studied. The results showed：with the preculture 3 d, OD₆₀₀ =0.8, infection time 10 min, cocultured for 3 d, 100 μmol/L acetosyringone conditions, the stable transformation rate of regenerated bulb scales could reach to the highest 12.78%; however, embryogenic callus preculture 2 d, OD₆₀₀= 0.8, infection time 10 min, cocultured 3 d, with 100 μmol/L acetosyringone conditions, which the stable transformation rate was the highest 12.22%. In all the conditions, the best hygromycinresistant screening concentrations always was 20 mg/L. PCR and reverse transcription PCR assay showed 9 putative transgenic plants were obtained. Southern hybridization analysisfurther proved 6 plants that the transgenic lilium flowers carry blue gene F3′5′H. The results provided technical support and material basis for the continuing development of novel bluish Lilium flowers.