Shoot apical bud cuttings of okra cultivar‘Qingfu’ in vitro (1.0 cm) were transplanted on MS basal medium supplemented with 0.05 g·L^-1 naphthalene acetic acid (NAA) and cultured under eight different quality lights: fluorescent lamp (CK), four mixture blue plus red light emitting diodes (BR_3∶1,BR_1∶1,BR_1∶3,BR_1∶4 LEDs), blue, red plus yellow (BRY=1∶3∶1) LEDs, blue, red plus green (BRG=1∶3∶1) LEDs, blue, red plus farred (BRFR=1∶3∶1) LEDs. The objective of present study was to determine the effects of different LEDs lights on the growth and physiological characteristics of okra (Abelmoschus esculentus) plantlets, and screen for optimal quality of okra plantlets in vitro in order to provide theoretical guidance and technical support of okra rapid propagation in vitro. The results showed that. (1) fresh mass, dry mass, root length, stem length and stem width were the greatest in plantlets cultured under BRY LEDs light; (2) root activity showed significantly higher in plantlets cultured under different lights than that of CK, and the highest was under BRY LEDs; Photosynthetic pigments were the highest in plantlets cultured under BRY LEDs, and which were also higher than that of other treatments; (3) the activities of SOD, POD and CAT were the greatest in plantlets cultured under BRY LEDs, and the lowest was that of CK; (4) the concentrations of soluble sugar, sucrose, soluble carbon and starch were the greatest in seedlings under BRY LEDs treatment, followed by BRG, and the lowest under CK; The concentrations of soluble protein, free amino acid and soluble nitrogen were the greatest in seedlings under BRY treatment, followed by BRG, and the lowest under CK. The present results indicated that larger and healthy plantlets and greater biomass of okra were produced in the presence of BRY (1∶3∶1) LEDs light. BRY light significantly promoted the root activity, photosythetic pigment content, antioxidant enzyme activities, carbon and nitrogen metabolism and accumulation of photosynthetic product of okra plantlets, which was the preferred light quality for the okra in vitro culture system.