NACs are one of the largest plantspecific transcription factor families. Members in this family play important roles in growth and abiotic stress responses. In this study, the fulllength cDNA sequence of CiNAC1 from Caragana intermedia was cloned by PCR technique. It was 1 066 bp in length. Bioinformatics analysis showed that CiNAC1 had an open reading frame of 921 bp, encoding 306 amino acids. The calculated molecular weight of CiNAC1 was 34.57 kD and the isoionic point was 8.35. The CiNAC1 protein was a hydrophilic protein which had a conserved NAM domain in the Nterminus. It contained 26 phosphorylation sites and 7 glycosylation sites. Realtime quantitative PCR analysis showed that CiNAC1 was induced by drought, NaCl, dehydration, and high pH. Localization assays revealed that CiNAC1 localized in the nuclei, consistent with its role as transcription factor. Overexpression of CiNAC1 promoted the lateral root formation and length of the primary root. These results indicated that CiNAC1 might be related to response to environmental stress in C. intermedia.