Here, the gene encoding DcCAD were cloned from carrot cultivar ‘Kurodagosun’ with RTPCR method. The length of DcCAD was 1 074 bp, and encoding 357 amino acids. The DcCAD may belong to hydrophilic protein. Phylogenetic analysis showed that DcCAD closed to CAD from tomato(XP_010314515.1). Quantitative realtime PCR analysis showed that the expression profiles of the DcCAD gene are significantly different in different tissues (root, leaf blade, and petiole) of carrot. Relative expression level of the DcCAD gene is leaf blade>root>petiole. The DcCAD gene is responsive to various abiotic stresses, such as heat (38 ℃), cold (4 ℃), drought (20% PEG) and salt (0.2 mol·L^-1 NaCl). The expression level was significantly increased after high temperature or low temperature treatments, respectively. The expression levels peaked at 1 h heat treatment and 2 h cold treatment, respectively. These results suggested that DcCAD gene participate in the response process of carrot under the abiotic stress.