Based on Chrysanthemum lavandulifolium RNAseq data, we obtained two code DNA sequences named ClHSP70 and ClHSP90 using RTPCR. Sequence analysis showed the predicted open reading frame (ORF) of ClHSP70 is 2 559 bp, encoding a protein of 852 amino acids. The conserver domain search exhibited that Nteminar contains typical NBD domain which belongs to HSP70 family. The predicted ORF of ClHSP90 is 2 094 bp, encoding a protein of 697 amino acids. The conserver domain search showed that Nteminar contains ATPasesuperfamily and HSP90 superfamily conservative structure. Bioinformatics showed that the amino acid sequences of ClHSP70 had high consistency with Glycine max and Nicotiana tomentosiformis HSP70, while ClHSP90 had high consistency with Ageratina adenophora HSP90. Realtime PCR demonstrated the expression patterns of ClHSP70 and ClHSP90 in leaves at 42 ℃. The expression of these two genes were both significantly upregulated after 0.5 h, reached the highest level after 1 h. Then the expression of both two genes decreased after 2 h, and remained at a relatively lower level after 4 h. In different plant tissues (root, stem, leaf) after 1 h at 42 ℃, ClHSP70 was highly expressed in mature leaves, lower in young, leaves, root, mature stems, young stems, and ClHSP90 was the highest in mature stems. The results showed that ClHSP70 and ClHSP90 participated the process of heat tolerance in Chrysanthemum lavandulifolium and laid a foundation for analyzing ClHSP70 and ClHSP90 gene function.