To study anthocyanin transport, we isolated a fulllength gene NtAN9 (GenBank Accession No. KX356542) by in silicon cloning and RTPCR method from Nicotiana tabacum. The NtAN9 contains a 690 bp open reading frame (ORF) encoding 229 amino acid residues, which belongs to a putative phi class glutathione stransferase (GST) family. Its genomic structure contains 3 exons and 2 introns. Multiple sequence alignment showed that NtAN9 has highest identities (88%) with petunia PhAN9 that is an anthocyanin transportrelated GST. Phylogenetic analysis showed that NtAN9 was clustered into an anthocyanin transportrelated GST group, and was an ortholog of PhAN11. The anthocyanin content analysis and realtime quantitation PCR (qRTPCR) showed that NtAN9 was expressed in different flower developmental stages, with highest level in stage Ⅲ, but almost no expression in root, stem and leaf which are almost no anthocyanin. These results suggest that the NtAN9 function is similar with PhAN9 and might be related to the transfer and accumulation of anthocyanin in tobacco plants.