A study was conducted to investigate the effects of different concentrations of Mn, Zn and Fe on variations of ergot alkaloids contentin symbiont of Epichlo gansuensisAchnatherum inebrians. The results showed that: (1) The contents of ergine and ergonovine under Mn²⁺ 5.0 mmol·L^-1 had a peak value at the 3rd week，which had significant(P<0.05) difference with other Mn²⁺ treatments, but the contents of ergonovine under 0.01 mmol·L^-1 had a peak value at the 6th and 9th week. (2) The contents of ergine had maximum under Zn²⁺ 7.0 mmol·L^-1 at the 3rd , 6th and 9th week , and the contents of ergine was significantly (P<0.05) higher at the 3rd week than those at the 6th and 9th week. (3) The contents of ergonovine had maximum under Fe²⁺ 21.0 mmol·L^-1 at the 3rd, 6th and 9th week. The contents of ergine had a peak value under 6.0～12.0 mmol·L^-1 at the 3rd, 6th and 9th week , and the contents of ergine was significantly (P＜0.05) higher at the 3rd week than those at the 6th and 9th week. The contents of ergonovine had maximum under Fe²⁺ 0.0 mmol·L^-1 at 3rd and 6th week, but under 0.03 mmol·L^-1 had a peak value at 9th week. So，high concentrations of Mn, Zn and Fe elements is helpful to stimulate the drunken horse grass infected by endophytic fungi to accumulate tow ergot alkaloid for a short time treatment.