To explore the phosphatesolubilizing ability of endophytic fungi and their plant growthpromoting potential, we evaluated the phosphatesolubilizing capability of endophytic fungi from ancient Sophora japonica leaves in Zhougongmiao by the method of dissolved phosphorus cycle and molybdenumbule, and detected the effects of inoculated sterile host plantlet on chlorophyll, soluble protein contents, SOD and POD activities. The results showed that, there were 28 out of 55 fungi strains from ancient S. japonica leaves with phosphatesolubilizing capability, and twelve strains among twentyeight endophytic fungi displayed strong capability of dissolving phosphate of Ca₃(PO₄)₂ by a test of phosphatesolubilizing capability, they were Pestalotiopsis sp.(ZG7), Fusarium sp.(ZG9, ZG23, ZG32, ZG36 and ZG53), Aspergillus sp.(ZG15 and ZG34), Alternaria sp.(ZG39 and ZG51), Trichoderma sp.(ZG42) and Epicoccum sp.(ZG48). Two strains with strong phosphatesolubilizing capability were then picked up, ZG15 and ZG34, belonged to Aspergillus spp..Their diameter of phosphorus solubilizing circle (d) and d/D (D, colony diameter) were both higher than that of others，and their solubilization of Ca₃(PO₄)₂ accounted for 1 238.28 mg·L^-1 and 941.22 mg·L^-1, respectively, which were significantly higher than that of others and control (P<0.01). Four strains (ZG7, ZG9, ZG15 and ZG48) from different genera were inoculated into aseptic S. japonica seedlings bottle to ensure their contact upon with each other. Mycelium was observed infecting into the cortex cells and around the surface epidemical cells of root tissue; under the treatment by Aspergillus niger ZG15, S. japonica seedlings chorophyll content, soluble protein content, SOD and POD net activities were all obviously higher than that of control (P<0.05), so, ZG15 could maintain healthy seedling growth and improve seedling resilience, which showed a great potential for ancient S. japonica protection and a potential application as an organismfertilizer for forest tree.