Mature leaves collected from Vitis davidii, V. amurensis, V. heyneana and V. chunganensis were used for chloroplast isolation and cpDNA extraction in this study. The two methods were the column plant chloroplast DNAout and modified highsalt lowpH method, and the results were compared with each other. (1) Both methods had separated the chloroplast of Chinese wild grapes, but the modified highsalt lowpH method obtained higher concentration and less impurity of chloroplast than that of column plant chloroplast DNAout. So the modified highsalt lowpH method was more suitable for chloroplast isolation. (2) The value of OD₂₆₀/OD₂₈₀ of cpDNA extracted by the column plant chloroplast DNAout was between 1.28 and 1.36, and the concentration was between 4.2 ng·μL^-1 and 7.8 ng·μL^-1, which did not meet the demand of subsequent chloroplast genome sequencing. In contrast, the value of OD₂₆₀/OD₂₈₀ of cpDNA extracted by the modified highsalt lowpH method was between 1.84 and 1.90 and the concentration was between 2 514.4 ng·μL^-1 and 4 133.7 ng·μL^-1, so the cpDNA extracted in this way was extremely highquality and pure. As a result, the cpDNA extracted by the modified highsalt lowpH method meet the demand of subsequent chloroplast genome sequencing. As a conclusion, the modified highsalt lowpH method isolated intact chloroplast and extract highquality cpDNA of Chinese wild grapes simply and quickly. And the cpDNA meet the demand of subsequent chloroplast genome sequencing. It was also a critical step to make further research of chloroplast genomes of Vitis L.