Cassava cultivar ‘Ku50’ was used as experiment materials in this study. A HDZip gene, MeHB2, was cloned from leaves of cassava ‘Ku50’ by RTPCR method. This gene contained 882 bp open reading frame (ORF) encoding 293 amino acids. Protein conserved domain prediction showed that MeHB2 protein contained several domains such as Homeodomain, Leu zipper and HDZip_N, indicating this gene belonged to members of HDZip II. Phylogenetic analysis exhibited that MeHB2 had close genetic relationship with its homologous genes in Jatropha curcas, Populus trichocarpa and Salix purpurea. Quantitative realtime PCR analysis revealed that low temperature, osmotic, ABA and H₂O₂ treatments significantly induced the expression of MeHB2, but salt stress depressed its expression. In addition, the expression of MeHB2 was also induced by shade stress. The results indicated that MeHB2 plays important roles in abiotic stress regulation in cassava.