To study the function of splicing factor responses to abiotic stress, a cDNA sequence of TaSF3B2 was isolated and analyzed from wheat EST database. The results showed that TaSF3B2 contained a 1 854 bp ORF encoding 617 amino acids. Using the methods of bioinformatics to predict and analyze TaSF3B2, the protein has classic domain of splicing factor 3B subunit 2, high hydrophilia (GRAVY, -0.895) and heat stability (Instability index, 43.92). The results of realtime PCR revealed that the expression of TaSF3B2 had different levels in different developments and tissues. In addition, the expression was induced markedly by drought, salt, lowtemperature, ABA and yellow rust (CY32)，with downregulation in leaves of seedling, but no change in root. In summary, it is speculated that TaSF3B2 could play a role not only in growth and development conditions but also in biotic and abiotic stresses of wheat.