Two truffles were taken as experimental materials which were from Helan mountain area in Inner Mongolia, and they were identified basing on the morphological and molecular methods. In the process of experiment, the DNA sequences were blasted using the GenBank, and phylogenetic trees were constructed by using Mega5.1 software. The results show that two strains of ascoma are hypogeous. Thereinto, ordera is sallow, subsphaeroidal, to irregular shape, and with microvilli on surface. And tuberb is yellowishbrown, uniform texture, with a wart on surface, and there is a special aroma of dried fruit. Through the analysis of molecular biological methods, ordera was identified as a species of Tuber genus, tuberb is Tuber brumale, and the tuberb is identified as a new record species in Inner Mongolia.