To study the effect of amino acid organic fertilizer and chemical fertilizer application for day matter accumulation, seed yield and quality of oil flax, without fertilizer as contrast under field conditions，we analyzed the single application of fertilizer, the proportion of different formula of amino acid organic manure and chemical fertilizers application and single application of amino acid organic fertilizer formula for flax. The results showed: (1) amino acid organic fertilizer had obvious effect for germination rate, and with the amino acid organic fertilizer increased the germination rate increased. (2) Amino acid organic fertilizer promoted the dry matter accumulation process and increased the total amount of dry matter accumulation of flax. In mature stage,30% organic fertilizer + 70% chemical fertilizer compared with no fertilizer, 100% chemical fertilizer and 100% organic fertilizer the total dry matter were significantly increased by 60.52%, 37.01% and 29.97%, respectively. Considering 30% organic fertilizer + 70% chemical fertilizer is the best effect, compared with no fertilization, 100% chemical fertilizer and 100% organic fertilizer, the yield increased by 72.07%, 16.47%, 13.30%, respectively. (3) In the case of 30% organic fertilizer + 70% chemical fertilizer alternative fertilizer, flax dry matter, seed yield and linolenic acid content is the highest, and 60% organic fertilizer + 40% chemical fertilizer alternative fertilizer, flax linoleic acid content is the highest.Considering the 30% organic fertilizer + 70% chemical fertilizer effect on local flax production of the best.