The plant calcium binding protein was found in pollen tube, which combines with Ca²⁺ directly or indirectly, orientates in membrane structure and forms Ca²⁺ signal channel. After activation of signal transmission, it will regulate pollen development and pollen tube growth. The calcium binding proteins CAM（calmodulin）,CDPK（Ca²⁺dependent kinases protein）,CML（calmodulinlike protein）,CBL（calcineurin Blike protein）and CIPK（CBLinteracting protein kinases） are recognized as key roles in the regulation of pollen development and pollen tube growth. In this review, the recent research of mechanism in different protein families and whose members between the various types of calcium binding are summarized. It also presented the effect on pollen tube specific mode of action and regulation with some examples from the calcium binding protein family members for varioustypes. In addiction, the forecast is discussed in the review about the future research in related fields.