Interactions between plant and environmental microorganism not only existed stress relationship but also possessed symbiotic relationship. Whether on plantpathogen interaction system caused damage to plant or on plant symbiotic system provided nutrition for plant, the regulation molecular mechanism of Ca²⁺ signaling in cell transduction pathways all played important roles. In this review, we focused on both interaction systems of regulation upstream mechanism of Ca²⁺ signaling in the cell transduction pathway in details.