4coumaric acid coenzyme A ligase is a key enzyme in lignin biosynthesis pathway. In this study, Pg4CL，the homologous gene of 4CL, was obtained with RACE and RTPCR method, with ‘Punica granatum cv. Hongyushizi’ as the test material. The cDNA fulllength sequence of Pg4CL was 2 121 bp, with reduced 544 amino acids. Sequence alignment and functional domain analysis revealed that Pg4CL contained two conserved domains: SSGTTGLPKGV and GEICIRG. Phylogenetic tree analysis indicated that Pg4CL shared the same origin with other species, and had the closest relationship with Eucalyptus camaldulensis Ec4CL. The fluorescent quantitative PCR analysis showed that Pg4CL expression was detected in leaves, peel, seed coat and stems. The expression in peel and leaves were higher, while in seed coat was the lowest; Pg4CL expression was detected in six cultivars of pomegranate seeds, and the expression of ‘Tunisiruanzi’ was higher, while ‘Huiliruanzi’ was lower; Pg4CL expression was detected in different development periods of ‘Fenpi’ seeds. The relative expression of Pg4CL gradually increased from 15 d-60 d, and reached a maximum at 60 d. While sharply declined from 75 d, and maintained a lower level of expression.