A AP2/ERF gene was isolated from Eremopyrum triticeum by RTPCR method, and designated as EtAP2 (GenBank accession no. KX622583). EtAP2 contained an open reading frame (ORF) of 1 128 bp encoding 375 amino acids. The molecular mass of EtAP2 was 40.87 kDa with isoelectric point (PI) of 5.36. Multiple sequences alignment and phylogenetic analysis indicated that EtAP2 protein contained two conserved AP2 domains, which had close genetic relationship with AP2/ERF transcription factor in Triticum aestivum. Quantitative realtime PCR analysis revealed that simulated drought stress (15% PEG6000) treatment significantly induced the expression of EtAP2, and root is more sensitive to drought stress than leaf. The results indicated than EtAP2 might involve in response to drought stress regulation in E.triticeum.