With tomato (Solanum lycopersicum L.) variety ‘Micro Tom’ as test materials, we cloned the tomato Calcineurin BLike gene (SlCBL1) from ‘Micro Tom’ fruitand constructed inplant expression vector with eGFP report gene. Then we analyzed the relationship between SlCBL1 gene overexpression and tomato fruit development. The results show that: (1) compared with nontransgenic plants and empty vector plant, overexpression SlCBL1 can promote fruit mature and made tomato fruit mature for 3 to 5 days in advance. (2) The expression of tomato fruit development related genes also regulated in different degrees. Including tomatoes mature in the process of pigment synthesis genes, the path of ethylene and ripe related transcription factors have been strongly regulated, and the expression level raised about 5 to 10 times compared with control. Results show that SlCBL1 gene can promote the fruit development, and by influencing the pigment synthesis genes and ripe related transcription factors to control tomato fruit mature.