In this study, Clematis ‘Ernest Markham’ which belongs to Lateflowering Clematis was chosen as the experimental material. The procedures of microsporogenesis and male gametophyte formation of Clematis ‘Ernest Markham’ were observed by traditional paraffin section technique, and we planned to explore its characteristics of embryonic development. The results were as follows: (1) each floret of inflorescence usually had dozens of fertile stamens, and the anther has four chambers. (2) The structure of anther wall was the monocotyledonous type, which was composed of epidermis, endothecium, middle layer and tapetum. (3) The cytokinesis of microspore mother cell meiosis was simultaneous type, and most of the tetrads were isobilateral, occasionally tetrahedral. (4) The mature pollen grains were the bicellular type with three grooves. With scanning electronic microscope, the mature pollen grains which appeared spherical in shape were observed, and the exine was almost smooth or wavy. These results suggested that microsporogenesis and male gametophyte development of Clematis ‘Ernest Markham’ were normal, which could be used as the male parental variety cultivating Clematis hybrid with high ornamental value and provided the basis for the further research about the embryological features of ornamental Clematis plants.