This study aimed to investigate the expression of Wdreb2 and Wlip19 in ten different varieties of wheat leaves under drought stress, and lay a foundation for wheat drought resistance mechanism. Primers were designed according to gene sequences, using wheat leaves under different degrees of drought stress (including 0,3,6,9,12 and 24 h) as experimental materials, and 26S rRNA as the internal control. The florescent realtime quantitative PCR was used to detect relative expression levels of Wdreb2 and Wlip19 in different varieties of wheat. RTPCR results showed that, the expression of Wdreb2 and Wlip19 gene in drought sensitivity wheat leaf significantly lower than that in drought tolerant wheat under drought stress. There are some differences in response time and express trends in different varieties. These results indicated that the different expression levels of Wdreb2 and Wlip19 in different varieties of wheat under drought stress were related to the drought resistant ability.