Flower bud of Cymbidium faberi was chosen as experiment material for gene cloning. A CINlike gene of TCP family was amplified through RTPCR technique and named as CfCIN (GenBank accession number KJ956809). The Open Reading Frame (ORF) of CfCIN is 1 161bp in length and encodes a protein with 386 amino acids. To investigate the specific functions of CfCIN, we constructed the expression vector and introduced it into Saintpaulia ionantha by Agrobacteriummediated method. Phenotypic analysis showed that the leaves of transgenic plants were bigger and changed from circular to oval. Margin of leaves changed from flat and smooth to slightly notched and back curly. Leaf veins were obvious and petioles were redder than wild type plants. There was no obvious difference in flower shape between the transgenic plants and wild type plants. These result indicated that CfCIN might participate leaf development.
Reference
Related
Cited by
Get Citation
LI Yuxia, YANG Yuxia, SUN Yuying, LIU Song, WANG Guangdong. Isolation and Functional Verification of CfCIN in Cymbidium faberi[J]. Acta Botanica Boreali-Occidentalia Sinica,2016,36(12):2354-2360