A complete CDS sequence of a glycogen synthase kinase family gene, JcGSK, was cloned from Jatropha curcas. JcGSK contained an opening reading frame (ORF) of 1 230 bp, and encoded 409 amino acids. The predicted molecular mass was 46.33 kD with theoretical isoelectric point(PI) of 8.58. Conserved domain analysis indicated that JcGSK had a typical domain of the GSK family. Multiple alignment and phylogenetic analysis showed that JcGSK had the highest identity(96%) and closest relationship with Hevea brasiliensis. Tissue expression results showed that JcGSK expressed significantly in different organisms and had the highest expression in root, NaCl, Abscisic acid (ABA), PEG, cold and mechanical damage upregulated JcGSK gene expression, indicated JcGSK involved in response abiotic stress and signal transduction process of J.curcas. JcGSK also highly expressed in seed. The expression level change of JcGSK in seed had the same trends with seed growth, indicated that JcGSK involved in regulation seed growth of J.curcas.