Using the homologybased cloning, we obtained a Fbox/LRR repeat protein 14 gene (TaFBL14) from wheat in the present study. TaFBL14 encoded a 486 aa polypeptide with predicted molecular weight of 53.48 kD and theoretical isoelectric point 5.93. The deduced protein included a Fbox domain in the Nterminal and 7 LRR domains in the Cterminal. No signal peptide and nuclear localization sequence were detected. Subcellular localization showed TaFBL14 mainly expressed in the cytoplasm. αhelix was the most important secondary structure, which made TaFBL14 the globular protein. Neighbourjoining tree showed that the deduced TaFBL14 protein shared high similarity with FBL14 from Aegilops tauschii and Triticum urartu. Quantitative reverse transcription PCR showed that TaFBL14 mainly expressed in wheat young leaves and flag leaves, and were upregulated expression from 6 hpi to 96 hpi. These results implied that TaFBL14 may be involved in wheat resistant to leaf rust pathogen infection.