In this study, we performed the genomewide identification of the KUP/HAK/KT family genes in soybean based on soybean genome sequence information. We also analyzed the gene character, protein structure, chromosome location, gene duplication and expression pattern of the family members. Our results showed that: (1) 30 KUP/HAK/KT transporter genes (GmHAK01-GmHAK30) had been identified in soybean genome, which were distributed on 15 chromosomes of soybean; The segmental duplication and tandem duplication were the contributors to the expansion of GmHAKs genes in soybean genome. (2) The sequence identity of GmHAKs was very high, and all of them had 12-14 transmembrane regions, and were located on the plasma membrane. (3) Phylogenetic analysis indicated that the GmHAKs in soybean can be classified into 4 clusters: ClusterⅠ-Ⅳ, and the number of ClusterⅡmembers was the most (16), and the number of Cluster Ⅳmembers was the least (1). (4) Gene structure analysis revealed that all GmHAKs genes had intron and exon. The intron number was between 7-9, and GmHAKs genes in the same subfamily exhibited similar exonintron pattern. (5) Expression pattern analysis showed that the expression of GmHAKs can be roughly divided into two categories: one is the expression of some tissuespecific genes, including all members of ClusterⅠand Cluster Ⅳ, part members of ClusterⅡ. These genes expressed highly in root (GmHAK30 and GmHAK04), or flower (GmHAK03 and GmHAK15), or pod (GmHAK10), or seed (GmHAK25); The other is the expression of some nontissue specific genes, including all members of Cluster Ⅲ, part members of ClusterⅡ, these genes (GmHAK05, GmHAK17 and GmHAK28) were highly expressed in all the examined tissues. The differential gene expression patterns of KUP/HAK/KT gene family in different evolutionary clusters suggested that it may be affected by selection in the process of evolution. The above results not only provide the important gene information for studying the KUP/HAK/KT gene function and the characteristics of potassium absorption in soybean, but also provides theoretical basis for the breeding of soybean varieties with high potassium efficiency.