Cutting seedlings of amur grape (V. amurensis Rupr. ) cultivars ‘Shuangyou’ (weak resistance) and ‘Zuoshanyi’ (strong resistance) were grown in plastic pots in shelter greenhouse. The effects of different salinealkali stress levels (light stress, moderate stress and severe stress) on photosynthetic characteristics and activities of photosystem Ⅱ (PS Ⅱ) in amur grape were studied using gas exchange and chlorophyll fluorescence induction dynamics analysis technology, and we surveyed their growth development. The results showed: (1) the net photosynthetic rate (P_n) and stomatal conductance (G_s) decreased significantly in lightly, moderately and severely stressed for ‘Shuangyou’ seedlings compared to control plants, but the transpiration rate (T_r) decreased significantly only under severe stress treatment. For ‘Zuoshanyi’ seedlings, P_n and T_r decreased significantly only under severe stress treatment, G_s decreased significantly under moderate and severe stress treatments, and intercellular CO₂ concentration (C_i) decreased significantly under different stress treatments. The reduction of P_n was mainly caused by stomatal limitation and nonstomatal limitation. (2) The specific energy fluxes for absorption (ABS/RS), the density of QA^- reducing PSⅡ reaction centers per cross section (RS/CS) and performance index (PI_ABS) decreased significantly in lightly and moderately stressed for ‘Shuangyou’ seedlings compared to control plants. For ‘Zuoshanyi’ seedlings, ABS/RS, RS/CS and the specific energy fluxes for electron transport (ET_o/_CS) decreased significantly only under severe stress treatment, other parameters under different stress treatments were similar to control plants. (3) Sap flow rate, plant height, leaf number and root biomass had significant decrease under different treatments, and decrease rate of ‘Shuangyou’ was higher. In conclusion, for ‘Zuoshanyi’ seedlings, salinealkali stress has relatively little effect on P_n, light energy absorption, electron transport efficacy and growth compared to ‘Zuoshanyi’, so ‘Zuoshanyi’ cultivar was more resistant to salinealkali than ‘Shuangyou’. For evaluation and detection of salinealkali tolerance in amur grape, we not only need to evaluate some physiological parameters such as photosynthetic function, but also to evaluate comprehensive growth indexes.