MS2 gene encodes fatty acyl reductase which involves in the fat metabolism of anther tapetum. In this study, with Ningxia wolfberry (Lycium barbarum L.) variety ‘Ningqi No.1’ as material, we obtained LbMS2 gene with RACE(rapidamplification of cDNA ends) method. The results indicated that: LbMS2 had an open reading frame (ORF) of 1 782 bp, which encoded a protein of 593 amino acid residues, and its isoelectric point was 8.98；Bioinformation analysis indicated that LbMS2 protein was located in the chloroplast；LbMS2 protein sequence showed the highest homology identity with MS2 protein from Petunia hybrida, Nicotiana tomentosiformis, Solanum tuberosum, Solanum lycopersicum and Capsicum annuum；Realtime PCR analysis indicated that LbMS2 gene was expressed in high transcript level in tetrad stage and single nucleus pollen stage of anther development. LbMS2 gene takes part in the flower development in wolfberry.