The epidermal morphology of eight species of Chamaesium was observed by light microscope and scanning electron microscope. The results indicated that: (1) the epidermal cells of eight Chamaesium species are irregular or regular polygon, with sinuolate and nearly straight anticlinal cells. The length to width ratio of upper epidermis cells was between 1.5 - 2.5. These were no stomatal in upper epidermis of three species, C. wolffianum, C. mallaeanum and C. viridiflorum. Stomatal density of other species was between 20 - 74/mm², and stomatal index was 6.0% - 17.7%. In the lower epidermis, all eight species have plenty of stomata, the stomatal density was 100 - 183/mm², stomatal index was between 16.1% - 23.6%. (2) C. paradoxum, C. novemjugum and C. spatuliferum were clustered in the first group, C. delavayi was formed the second group, C. wolffianum, C. mallaeanum and C. viridiflorum belonged to the third group, while C. thalictrifolium formed an individual group by cluster analysis, which is almost accordance with the characteristics of leaf epidermis. (3) Characteristics of leaf epidermis play important roles in taxonomy of Chamaesium.