A gene encoding UDPglycose: flavonoid glycosyltransferase (UFGT) was isolated from peels of Punica granatum L. with RTPCR and RACE methods. The accession number in GenBank was KF841620.The length of OFR was 1 476 bp, encoding 491 amino acids. The deduced PgUFGT protein contained a typical conserved PSPG motif, an UDPglucosyltransferase and UDPglucoronosyltransferase/glucosyltransferase (UDPGT) domain, showing highly identities to UFGTs of other plants. Phylogenetic tree analysis showed that PgUFGT belonged to flavonoid 3Oglycosyltransferase categories. The results from qRTPCR indicated that the expression patterns of PgUFGT were different in Hongbaoshi and Shuijingtian cultivars during fruit development. In Hongbaoshi pomegranate, the transcription abundance of PgUFGT had two peaks, while in Shuijingtian pomegranate, only one expression peak of PgUFGT was observed.The roles of PgUFGT played may be different in two pomegranate cultivars. These results were the basis of further studies on molecular mechanisms of pomegranate fruit coloration.